Category: collagen 17
implications for the immunotherapy of cancer
Background: Interleukin-15 (IL-15) is an important cytokine necessary for proliferation and maintenance of natural killer (NK) and CD8+ T cells, and with great promise as an immuno-oncology therapeutic. However, IL-15 has a very short half-life and a single administration does not provide the sustained exposure required for optimal stimulation of target immune cells. The purpose of this…
Read Morekidney releases a non-polymerizing form of Uromodulin in the urine and circulation that retains the external hydrophobic patch
Processing of mature urinary THP, which polymerizes into supra-molecular filaments, requires cleavage of an external hydrophobic patch (EHP) at the C-terminus. However, THP in the circulation is not polymerized, and it remains unclear if non-aggregated forms of THP exist natively in the urine. We propose that an alternative processing path, which retains the EHP domain, can lead…
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Chromatin immunoprecipitation (ChIP)
Background Knowledge Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount…
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Cell Counting Package deal-8
Choices & Properties Choices Fast and useful Simple one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your total assay shall be carried out straight in…
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Background staining or non-specific staining buster peptide blocker
INTRODUCTION Background staining or non-specific staining is an often-encountered disadvantage in immunohistochemistry (IHC), in immunofluorescence labeling (IF) and in situ stains. Background staining is attributable to varied parts equivalent to cross reactivity of antibodies with the shared epitopes throughout the tissue, by the presence of pure and/or contaminating antibodies present throughout the main antibody and/or…
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