Tag: recombinant dna technology
implications for the immunotherapy of cancer
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Background: Interleukin-15 (IL-15) is an important cytokine necessary for proliferation and maintenance of natural killer (NK) and CD8+ T cells, and with great promise as an immuno-oncology therapeutic. However, IL-15 has a very short half-life and a single administration does not provide the sustained exposure required for optimal stimulation of target immune cells. The purpose of this…
Read MoreChromatin immunoprecipitation (ChIP)
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Background Knowledge Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount…
Read MoreCell Counting Package deal-8
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Choices & Properties Choices Fast and useful Simple one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your total assay shall be carried out straight in…
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