Tag: western blot method
kidney releases a non-polymerizing form of Uromodulin in the urine and circulation that retains the external hydrophobic patch
- Bailey
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Processing of mature urinary THP, which polymerizes into supra-molecular filaments, requires cleavage of an external hydrophobic patch (EHP) at the C-terminus. However, THP in the circulation is not polymerized, and it remains unclear if non-aggregated forms of THP exist natively in the urine. We propose that an alternative processing path, which retains the EHP domain, can lead…
Read MoreCell Counting Package deal-8
- Bailey
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Choices & Properties Choices Fast and useful Simple one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your total assay shall be carried out straight in…
Read MoreBackground staining or non-specific staining buster peptide blocker
- Bailey
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INTRODUCTION Background staining or non-specific staining is an often-encountered disadvantage in immunohistochemistry (IHC), in immunofluorescence labeling (IF) and in situ stains. Background staining is attributable to varied parts equivalent to cross reactivity of antibodies with the shared epitopes throughout the tissue, by the presence of pure and/or contaminating antibodies present throughout the main antibody and/or…
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