Background staining or non-specific staining buster peptide blocker
INTRODUCTION
Background staining or non-specific staining is an often-encountered disadvantage in immunohistochemistry (IHC),
in immunofluorescence labeling (IF) and in situ stains. Background staining is attributable to varied parts
equivalent to cross reactivity of antibodies with the shared epitopes throughout the tissue, by the presence of pure and/or contaminating antibodies present throughout the main antibody and/or the secondary antibody,by ionic interactions, by the presence of carbohydrates and by endogenous biotin present throughout the tissue. Eradicating background is most significant for buying background-free specific staining for the good thing about qualitative and quantitative evaluation.
PRODUCT DESCRIPTION
Innovex Background Buster is a peptide Blocker that eradicates all frequent background staining.
Background Buster removes all background staining attributable to main antibodies, by secondary staining
reagents, by chromogens, by fixatives, by extreme heat retrieval (HIER) and by endogeneouse biotin present in
tissues equivalent to liver, pleen and kidney. Background Buster is used as a substitute of normal sera and completely different blocking decision for eradicating background staining in every human and animal tissues.
Innovex Background Buster is related to IHC staining, to immunofluorescence staining and to in situ probe
staining in every human and animal tissues. It’s often related to flow into cytometric assays.
Innovex Background Buster is a ought to for animal tissue staining, it is notably essential when staining
equal species tissue and antibodies equivalent to mouse antibodies on mouse tissues (Mouse-on-Mouse) and Rabbit-on- Rabbit. A 30-minute incubation with Innovex Background Buster is helpful earlier to the
utility of the primary antibody for staining of equal species main antibodies and tissues equivalent to
mous-on-mouse.
The utilization of Background Buster is extraordinarily useful for staining of indirect species (non-identical species
tissue/ antibody) equivalent to Rat –On-Mouse, Mouse-on- Rat, Mouse-on-Rabbit, and so forth. A 20-minute incubation with Innovex Background Buster is helpful earlier to the equipment of the primary antibody for staining of Indirect (non-identical species main antibodies and tissues). In immunoperoxidase-IHC staining, one different kind of background and non-specific staining is attributable to pink blood cell staining; that is due to endogenous peroxidase enzyme present in pink blood cells. This type of background requires a pre-treatment step with 3% freshly made hydrogen peroxide (H2O2) in water or use of INNOVEX STABLE PEROXIDE BLOCK. this blocking step must precede the blocking step with Innovex Background Buster.
Background Buster could be utilized for IHC Background or non-specific staining is often seen in numerous immunoassays, in immunohistochemistry IHC) and completely different immunoassay varieties equivalent to immunofluorescence, ELISA and flow into cytometric assays, background staining will likely be prevented by means of INNOVEX peptide Blocker know-how, Background Buster.
Innovex Background Buster is a varied and extremely efficient Peptide-based Know-how for eliminating frequent background staining in tissues and cell preparations stained with antibodies in immunohistochemistry or with DNA or RNA probes for in-situ procedures.
It moreover makes background-free staining of mouse antibodies on mouse tissues attainable in IHC &IF.staining.
Background Buster may also be related to eliminating non-specific binding in immunofluorescence, ELISA and flow into cytometric assays.
ADVANCED AND UNIQUE FEATURES
- Permits staining of equal species antibodies and tissues (e.g., mouse antibody on mouse tissue, rat-on-rat, rabbit-on-rabbit, and so forth.).
- Fast 10 minute incubation step earlier to creating use of main antibody or in-situ probe at room temperature
- Delivers full eradication of frequent background staining
- Replaces utilizing common serum, powdered milk, casein, and completely different blocking brokers and renders full success
- Great for every frozen and paraffin sections
- Great for in situ utility
- A ought to for animal tissue staining.
Spectroph Background Sol. |
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| OPSB | Scientific Laboratory Supplies | 125ML | EUR 86.17 |
DAB Liquid/Substrate (stable), Background-free; 2- |
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| NB314SBD | Innovex | 70 ml | EUR 295 |
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Description: DAB Liquid/Substrate (stable), Background-free; 2-component system, 70 ml (Large volume) |
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DAB Liquid/Substrate (stable), Background-free; 2- |
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| NB314SBD-35 | Innovex | 35 ml | EUR 225 |
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Description: DAB Liquid/Substrate (stable), Background-free; 2-component system, 35 ml (Small volume) |
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TrueBlack IF Background Suppressor System (Permeabilizing) |
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| 23012 | Biotium | 200AS | EUR 319.2 |
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Description: Minimum order quantity: 1 unit of 200AS |
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TrueBlack IF Background Suppressor System (Permeabilizing) |
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| 23012-T | Biotium | 20AS | EUR 111.6 |
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Description: Minimum order quantity: 1 unit of 20AS |
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Sample, Antibody and Conjugate Diluent (20X) for ELISA (For serum, plasma sample conservation and low background) |
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| 80066 | Alpha Diagnostics | 50 ml | EUR 278.4 |
NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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| abx235887-100ug | Abbexa | 100 ug | EUR 577.2 |
NEDD8 ultimate buster 1 (NUB1) Antibody |
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| abx331552-100ul | Abbexa | 100 ul | EUR 510 |
NEDD8 ultimate buster 1 (NUB1) Antibody |
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| 20-abx326307 | Abbexa |
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NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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| 20-abx124654 | Abbexa |
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NEDD8 Ultimate Buster 1 (NUB1) Antibody |
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| 20-abx008140 | Abbexa |
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NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18 |
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| F54770-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
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Description: NUB1/NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. |
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NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18 |
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| F54770-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
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Description: NUB1/NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. |
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Mouse NEDD8 ultimate buster 1 (NUB1) ELISA Kit |
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| abx390078-96tests | Abbexa | 96 tests | EUR 1093.2 |
Human NEDD8 ultimate buster 1 (NUB1) ELISA Kit |
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| abx381904-96tests | Abbexa | 96 tests | EUR 1093.2 |
Mouse NEDD8 ultimate buster 1, Nub1 ELISA KIT |
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| ELI-44223m | Lifescience Market | 96 Tests | EUR 1038 |
Human NEDD8 ultimate buster 1, NUB1 ELISA KIT |
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| ELI-44504h | Lifescience Market | 96 Tests | EUR 988.8 |
Bovine NEDD8 ultimate buster 1, NUB1 ELISA KIT |
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| ELI-13766b | Lifescience Market | 96 Tests | EUR 1113.6 |
Nub1 ELISA Kit| Mouse NEDD8 ultimate buster 1 ELISA Kit |
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| EF015717 | Lifescience Market | 96 Tests | EUR 826.8 |
