Epigentek

Chromatin immunoprecipitation (ChIP)

Background Knowledge
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly in demand.

Background Knowledge

Chromatin immunoprecipitaton (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this package deal will be utilized in numerous chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin can even be utilized in several chromatin-related capabilities equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Useful and versatile: the package deal is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

 

epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

 

Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Bundle accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

gPURE EXTRACTION KIT

IB47430 3ML
EUR 30.42
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT

IB47431 100ML
EUR 196.06
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT

IB47432 1000ML
EUR 1358.49
Description: (FOR CULTURED CELLS)

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

FFPE DNA Extraction Kit

EP016 50T
EUR 140

Cell DNA Extraction Kit      

TBS6007 1000 samples
EUR 258

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Total RNA Extraction Kit

EP014 50T
EUR 164

VIRAL DNA EXTRACTION KIT

IB47400 4 PREPS
EUR 30.11

VIRAL DNA EXTRACTION KIT

IB47401 50 PREPS
EUR 238.33

VIRAL DNA EXTRACTION KIT

IB47402 100 PREPS
EUR 428.75

VIRAL DNA EXTRACTION KIT

IB47403 300 PREPS
EUR 1151.98

Blood DNA Extraction Kit   

TBS6004 1000 samples
EUR 298

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
EUR 418.09

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50 each
EUR 548.4

Exosomal RNA Extraction Kit

P200R 20 rxn
EUR 389

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Total Protein Extraction Kit

K3011010 1 kit
EUR 367

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

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