Chromatin immunoprecipitation (ChIP)

• Bailey
• December 1, 2021
• 0

Background Knowledge
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly in demand.

Background Knowledge

Chromatin immunoprecipitaton (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this package deal will be utilized in numerous chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin can even be utilized in several chromatin-related capabilities equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Useful and versatile: the package deal is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 10⁵ to 5 x 10⁶ cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 10⁶ cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 10⁶  cells or per 50 mg tissues.

ExoDNA? Extraction Kit
K1242-25	Biovision		EUR 588

ExoDNA? Extraction Kit
K1242-50	Biovision		EUR 881

miRNA Extraction Kit
K1456-50	Biovision	50 Preps	EUR 551

Total Protein Extraction Kit
BSP003	Bio Basic	50Preps	EUR 109.16

Plant Protein Extraction Kit
BSP004	Bio Basic	20Preps	EUR 110.9

Nuclear Protein Extraction Kit
BSP009	Bio Basic	50Preps	EUR 89.15

FFPE Protein Extraction Kit
BSP058	Bio Basic	50Preps	EUR 102.2

EpiQuik Nuclear Extraction Kit
OP-0002	EpiGentek	100 Assays	EUR 292.15
Description: kits suitable for this type of research

Quick Gel Extraction Kit
20-abx098085	Abbexa	EUR 286.00 EUR 203.00	200 rxns 50 rxns

Up RNA Extraction Kit
20-abx098087	Abbexa	EUR 258.00 EUR 105.00	100 ml 3 ml

Plant RNA extraction Kit
20-abx098088	Abbexa	EUR 258.00 EUR 105.00	100 ml 3 ml

Exosomal RNA Extraction Kit
P200R,-part-of-P200	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-25	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-50	101Bio	-	Ask for price

Membrane Protein Extraction Kit
K2113-50	ApexBio	50 assays	EUR 544

Mammalian Cell Extraction Kit
K2114-500	ApexBio	500 assays	EUR 321

Membrane Protein Extraction Kit
K268-50	Biovision		EUR 539

Mammalian Cell Extraction Kit
K269-500	Biovision		EUR 294

Plant Tissue Extraction Kit
K296-50	Biovision		EUR 457

Bacterial RNA Extraction Kit
R403-01	Vazyme	100 rxn	EUR 155

Membrane Protein Extraction Kit
55R-1357	Fitzgerald	50 assays	EUR 740
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit
55R-1358	Fitzgerald	500 assays	EUR 381
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Total RNA Extraction Kit
K2014005	Biochain	1 kit	EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein Extraction Kit
K3011010	Biochain	1 kit	EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit
K3014005	Biochain	1 kit	EUR 382
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Genomic DNA Extraction Kit
K5016005	Biochain	1 kit	EUR 329

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-100	GenDepot	100ml	EUR 126

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-500	GenDepot	500ml	EUR 142

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Cell Counting Package deal-8

Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric)