Detection Kit for Monkeypox Virus DNA
Polymerase chain reaction (PCR) is the preferred laboratory test given its accuracy and sensitivity suggested by the WHO. PCR test confirmation for monkeypox virus infection is based on nucleic acid amplification test (NAAT), use of real-time polymerase chain reaction (PCR) is preferred or conventional such as the Monkeypox Detection Kit for the detection of unique viral DNA sequences, and can be used alone.
Monkeypox Test Kit Features
- Reliable: Based on the WHO-recommended diagnostic method: real-time polymerase chain reaction (PCR)
- High specificity: highly conservative region of the coding region of the monkeypox virus gene
- High precision: Endogenous internal involved to monitor the entire NAAT procedure from sampling to PCR result
- Universal: compatible with a conventional real-time fluorescent PCR system.
Monkeypox Diagnostic Kit
Test principle: real-time fluorescent PCR
Type of sample
Rashes, scabs, blister fluid, pustular fluid, or whole blood samples
FAM: Monkeypox conservative region, VIC: endogenous internal control
LOD: 200 copies/mL
Storage and shelf life: -20±5℃, 12 months
Applicable instruments: ABI 7500, AGS4800, AGS8830
Monkeypox Test Kit Procedure
- Sample collection: Take the rash cover or scab, put it into a sample collection tube, then seal the tube and send it for inspection.
- Sample processing and nucleic acid extraction: use of nucleic acid extraction kit and instrument to obtain purified MPOX DNA.
- PCR amplification: preparation of PCR reagents and use of extracted MPOX nucleic acid as PCR template
- Analysis of results